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Determination of cortisol in human plasma by stable isotope dilution mass spectrometry
Author(s) -
Kasuya Yasuji,
Furuta Takashi,
Hirota Noriko
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200160159
Subject(s) - isotope dilution , chemistry , chromatography , mass spectrometry , reproducibility , dilution , isotope , gas chromatography–mass spectrometry , gas chromatography , human plasma , capillary action , coefficient of variation , stable isotope ratio , plasma , accuracy and precision , analytical chemistry (journal) , materials science , physics , quantum mechanics , thermodynamics , statistics , mathematics , composite material
Cortisol selectively labelled with 2 H at the 19‐methyl and the C‐1 position (cortisol‐ d 5 ) was synthesized and used as an internal standard in stable isotope dilution mass spectrometry in order to determine cortisol in human plasma. A capillary gas chromatographic/mass spectrometric method provided a sensitive and reliable technique with good accuracy, precision and reproducibility without complex corrections for contributions by using cortisol‐ d 5 ( m / z 605 and 610, respectively). The sensitivity of the gas chromatographic/mass spectrometric assay was 1.02 ng per injection, with a signal‐to‐noise ratio of about 6. The inter‐ and intra‐assay coefficients of variation for plasma sample were 3.07% and 1.77%, respectively.