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Particular matrix for fast atom bombardment mass spectrometric analysis of phenolic glycolipid antigens isolated from pathogen mycobacteria
Author(s) -
Rivière M.,
Fournié J. J.,
Vercellone A.,
Puzo G.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200160153
Subject(s) - fast atom bombardment , glycolipid , glycerol , chemistry , pathogen , molecule , matrix (chemical analysis) , mass spectrometry , antigen , stereochemistry , biochemistry , chromatography , microbiology and biotechnology , organic chemistry , biology , genetics
Phenolic glycolipids play a key role as an antigenic probe for serodiagnosis of some human pathogen mycobacterial infections. The lipidic part which corresponds to a phenolphthiocerol dimycocerosate molecule, and the presence of partial O ‐methylated sugars, confer a high hydrophobicity to this kind of molecule. Fast atom bombardment (FAB) mass spectrometric analysis with standard matrices such as glycerol or thioglycerol was unsuccessful. Using a new matrix—monobutyltriethylene glycol—FAB analysis allows molecular weight determination and partial structrual elucidation of the saccharidic and the lipidic part of those compounds.