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Analysis of lyso ‐platelet activating factor by negative ion gas chromatography/mass spectrometry of the nitrobenzal acetal derivatives
Author(s) -
Mallet A. I.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200160137
Subject(s) - chemistry , acetal , mass spectrometry , alkyl , chromatography , derivative (finance) , platelet activating factor , metabolite , gas chromatography–mass spectrometry , glycerol , gas chromatography , electron ionization , hydrolysis , ion , organic chemistry , biochemistry , ionization , medicine , financial economics , economics
This paper describes the methods that have been employed for the quantification of platelet activating factor (PAF) and its principal metabolite, in biological matrices. In plasma PAF is rapidly hydrolysed to lyso ‐PAF, which is also the major precursor of PAF. Measurement of lyso ‐PAF has been accomplished by mass spectrometric methods using cyclic acetal derivatives of the alkyl glycerol produced after removal of the polar head group. We describe the preparation of a novel electron capture derivative for this glycerol and its behaviour under electron impact and negative ion mass spectrometry. High sensitivities have been achieved using the procedures described here.