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In‐depth limitation of the Lamma 500 for the in situ localization of organic compounds in biological embedded tissue samples
Author(s) -
Van Vaeck L.,
Van Espen P.,
Jacob W.,
Gijbels R.,
Cautreels W.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200160120
Subject(s) - microprobe , microcrystalline , in situ , materials science , analytical chemistry (journal) , mass spectrometry , ion , layer (electronics) , calibration , resolution (logic) , chemistry , nanotechnology , chromatography , mineralogy , physics , crystallography , organic chemistry , quantum mechanics , artificial intelligence , computer science
The objective of this study was exploration of the potential, offered by the laser microprobe mass spectrometer (LAMMA), for the in situ localization of organic targets in embedded tissues by means of structurally relevant ions. A series of model systems was designed to evaluate stepwise the analytical problems involved. A preliminary screening pointed to the position of the target in comparison to the actual sample surface as a determining parameter. Refined simulations were carried out with sandwich samples, consisting of an epon carrier (thickness 1 μm), coated with microcrystalline targets and covered with a layer between 5 and 50 nm of different materials (epon, carbon, formvar). In addition to a stimulated conversion of molecular into fragment ions, the presence of a barrier leads to a drastic loss of sensitivity (20–50 x) and unacceptable degradation of the mass spectrometric quality (resolution, calibration).