On‐line fast atom bombardment analysis of dynamic biological systems | Zendy

Caprioli Richard M. | Zendy

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On‐line fast atom bombardment analysis of dynamic biological systems

Author(s) -

Caprioli Richard M.

Publication year - 1988

Publication title -

biomedical and environmental mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0887-6134

DOI - 10.1002/bms.1200160107

Subject(s) - substrate (aquarium) , continuous flow , chemistry , flow (mathematics) , aqueous solution , kinetics , hydrolysis , fast atom bombardment , analytical chemistry (journal) , flow injection analysis , sequence (biology) , enzyme kinetics , enzyme , materials science , biological system , combinatorial chemistry , chromatography , active site , physics , biochemistry , mechanics , mass spectrometry , oceanography , quantum mechanics , detection limit , biology , geology

The continuous‐flow fast atom bombardment probe interface has been used for the direct analysis of aqueous biochemical reactions. This device was used in two ways: in a constant flow mode and a flow injection mode. Each method has particular advantages which are illustrated with specific applications, including measurement of enzyme kinetics, comparative rates of hydrolysis of multi‐substrate/single enzyme reactions, C‐terminal peptide sequence analysis, and characterization of multi‐enzyme/single substrate systems.

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