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Self‐chemical ionization Fourier transform ion cyclotron resonance mass spectrometry: Identification and characterization of modified and unmodified bases and nucleosides
Author(s) -
Weller Robert R.,
Mayernik Janet A.,
Giam C. S.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200151004
Subject(s) - fourier transform ion cyclotron resonance , chemistry , mass spectrometry , ion cyclotron resonance , adduct , protonation , analytical chemistry (journal) , ionization , chemical ionization , ion , tandem mass spectrometry , electrospray ionization , resolution (logic) , mass spectrum , protein mass spectrometry , chromatography , organic chemistry , cyclotron , artificial intelligence , computer science
Self‐chemical ionization Fourier transform ion cyclotron resonance (FT‐ICR) mass spectra are reported for bases, nucleosides, and alkylated and exocyclic adducts of bases and nucleosides. The technique always produces a protonated molecular ion and in the majority of cases this is a single, intense peak. Analysis of a base mixture and a nucleoside mixture demonstrates the technique as an excellent method to identify the constituent compounds qualitatively. The high resolution capabilities and tandem mass spectrometric techniques (ms n ) in FT‐ICR are discussed with respect to developing the technique as a future method to identify and characterize nucleic acid constituents, specifically adducts.