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Characterization of doxylamine and pyrilamine metabolites via thermospray/mass spectrometry and tandem mass spectrometry
Author(s) -
Korfmacher W. A.,
Holder C. L.,
Betowski L. D.,
Mitchum R. K.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150907
Subject(s) - pyrilamine , chemistry , thermospray , mass spectrometry , tandem mass spectrometry , chromatography , metabolite , selected reaction monitoring , biochemistry , receptor , antagonist
Analysis of doxylamine N ‐oxide and pyrilamine N ‐oxide as synthetic standards and biologically derived metabolites by thermospray mass spectrometry (TSP/MS) provided [M + H] + ions for each metabolite. TSP/tandem mass spectrometry (TSP/MS/MS) of the [M + H] + ions provided fragment ions characteristic of these metabolites. In addition, TSP mass spectrometry and TSP/MS/MS analysis of ring‐hydroxylated N ‐desmethyldoxylamine, N ‐desmethylpyrilamine and O ‐dealkylated pyrilamine is also reported. A fragmentation pathway for analysis by MS/MS of pyrilamine and its metabolites is also proposed. The results demonstrate the utility of TSP/MS for biologically derived metabolites of pyrilamine and doxylamine.