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Distinction of α‐ and β‐aspartyl and α‐ and γ‐glutamyl peptides by fast atom bombardment/tandem mass spectrometry
Author(s) -
Lloyd J. R.,
Cotter M. L.,
Ohori D.,
Doyle D. L.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150707
Subject(s) - fast atom bombardment , chemistry , tandem mass spectrometry , mass spectrometry , tandem , ion , fragment (logic) , atom (system on chip) , analytical chemistry (journal) , chromatography , materials science , organic chemistry , computer science , embedded system , composite material , programming language
A fast atom bombardment/collisional activation/linked‐scan at constant B / E (tandern mass spectrometric) method is described which can distinguish between α‐ and β‐aspartyl and α‐ and μ‐glutamyl underivatized peptides. The method is based upon differences in loss of CO from aspartyl or glutamyl B‐fragment ions (I B ) in these isomers which are rationalized from the stability of the resultant A‐fragment ions (I A ). It was observed that the ratio of I B :I A which was used in this determination was dependent upon the collision cell pressure. The higher the collision cell pressure, the larger the difference between the I B : I A ratios for these linkages.