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Identification of a biliary metabolite of cisapride
Author(s) -
Lauwers W.,
Le Jeune L.,
Meuldermans W.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150604
Subject(s) - metabolite , cisapride , chemistry , thermospray , mass spectrometry , fast atom bombardment , chromatography , pharmacology , biochemistry , medicine , tandem mass spectrometry , selected reaction monitoring
Radiolabeled cisapride was administered orally to male Wistar rats. The drug was metabolized extensively, resulting in the formation of a large number of urinary and faecal metabolites. In bile‐cannulated rats a major metabolite was excreted with the bile whose structure could not be elucidated with the aid of the registered electron impact and desorption chemical ionization spectra. Therefore the biliary metabolite was subjected to extensive analytical procedures combining fast atom bombardment mass spectrometry, thermospray liquid chromatography/mass spectrometry, nuclear magnetic resonance and ultraviolet analysis. The results of this study allowed the identification of the biliary metabolite as the O ‐sulphate of metabolically formed 3′‐hydroxycisapride.