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Laser desorption/fourier transform ion cyclotron resonance mass spectrometry: Digoxin, digitoxin, and their reduced and sugar‐hydrolyzed metabolites
Author(s) -
Shomo Ronald E.,
Chandrasekaran Appavu,
Marshall Alan G.,
Reuning Richard H.,
Robertson Larry W.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150510
Subject(s) - fourier transform ion cyclotron resonance , digitoxin , chemistry , mass spectrometry , analytical chemistry (journal) , mass spectrum , ion , digoxin , ion cyclotron resonance , ion source , chromatography , cyclotron , heart failure , medicine , organic chemistry
Mass spectra of digoxin and digitoxin (the most widely prescribed drugs for treatment of congestive heart failure) and a complete set of their 14 dihydro‐ and sugar‐hydrolyzed metabolites have been obtained via laser desorption/ionization with a Fourier transform ion cyclotron resonance (LD/FT/ICR) mass spectrometer. The most intense peak is typically the pseudomolecular [M + K] + ion, but fragment ions corresponding to loss of 1‐‐3 sugars and hydroxyls are also observed. LD/FT/ICR mass spectra for all 16 compounds were produced with a single set of sample and spectrometer parameters. No matrix peaks are present. Finally, LD/FT/ICR provides dynamic mass accuracy within ≈5 ppm throughout a mass range of 404 < m / z < 819.