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Comparison of methanol thermospray (filament on) and direct chemical ionization mass spectrometry to the study of biologically active steroids
Author(s) -
Das P. R.,
Pramanik B. N.,
Malchow R. D.,
Ng K. J.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150504
Subject(s) - thermospray , chemistry , ammonium acetate , chemical ionization , mass spectrometry , methanol , mass spectrum , pyridine , selectivity , chromatography , ionization , organic chemistry , high performance liquid chromatography , ion , selected reaction monitoring , tandem mass spectrometry , catalysis
A series of biologically active steroids was analyzed by both, ‘filament on’ thermospray using 100% methanol as the mobile phase with ammonium acetate as buffer, and direct chemical ionization with NH 3 gas. The mass spectra obtained were very similar. Addition of pyridine instead of ammonium acetate showed selectivity in adding to the steroid molecule.