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Positive ion fast atom bombardment mass spectrometry of reductively pyridylaminated maltooligosaccharides and its application to α‐amylase assay
Author(s) -
Honda Susumu,
Kakehi Kazuaki,
Ohmura Takeshi,
Morita Masanori
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150408
Subject(s) - fast atom bombardment , chemistry , ion , amylase , mass spectrometry , enzyme , chromatography , biochemistry , organic chemistry
Positive ion fast atom bombardment mass spectra of maltooligosaccharides reductively aminated with 2‐aminopyridine (G n ‐AP) contain abundant [M + H] + ions. Determination of G2‐AP and G3‐AP produced from G5‐AP by the action of α‐amylases, based on the abundance of their [M + H] + ions relative to that of cellobiose reductively aminated with 2‐amino‐6‐methylpyridine as the internal standard, allowed rapid and reproducible assay of these enzymes. It was advantageous for clinical investigation that the proportion of pancreatic and salivary α‐amylase activities could be determined.