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Mass spectral investigations on trichothecene mycotoxins. V. Direct analysis of satratoxins by tandem mass spectrometric techniques
Author(s) -
Krishnamurthy Thaiya,
Sarver Emory W.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150402
Subject(s) - trichothecene , chemistry , protonation , tandem mass spectrometry , tandem , fermentation , dissociation (chemistry) , mycotoxin , mass spectrometry , chromatography , ion , molecule , chemical ionization , analytical chemistry (journal) , ionization , organic chemistry , food science , materials science , composite material
Under chemical ionization conditions, satratoxins preferentially produce negatively charged molecular ions (M − ˙) over the positively charged protonated molecules. Collisionally activated dissociation of the M − ˙ ions resulted in daughter ions and neutral losses which were characteristic of the macrocyclic ester bridges. Direct tandem mass spectrometric methods of analyses wee developed and applied for the detection and quantification of satratoxins in Stachybotrys atra fermentation broths. Minimum detectable levels for satratoxin standards were 5 pg. A synthetically modified macrocyclic trichothecene, 8‐ketoverrucarin A, was used as an internal standard for the quantification of satratoxins in fermentation samples.