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Electron capture negative ion chemical ionization analysis of arachidonic acid
Author(s) -
Hadley Jean S.,
Fradin Armel,
Murphy Robert C.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150309
Subject(s) - glycerophospholipid , arachidonic acid , glycerophospholipids , chemistry , chromatography , mass spectrometry , chemical ionization , electron ionization , ionization , ion , yield (engineering) , organic chemistry , biochemistry , materials science , enzyme , phospholipid , membrane , metallurgy
A highly sensitive (subpicomole level) and structural specific method for the analysis of arachidonic acid esterified to complex glycerophospholipids has been developed using combined capillary gas chromatography and mass spectrometry. The methodology is based upon the formation of the pentafluorobenzyl ester of arachidonic acid, which is efficiently ionized using electron capture negative ion chemical ionization conditions to yield an abundant carboxylate anion at m / z 301. Quantification is carried out following hydrolysis of the complex glycerophospholipid in the presence of a known amount of ( 2 H 8 ) araachidonic acid. The use of this method is illustrated by the quantification of arachidonic acid within the glycerophospholipid classes isolated from resident peritoneal macrophage cells isolated from HS mice.