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Evaluation of the quantitative analysis of a steroid sulphate using fast atom bombardment and tandem mass spectrometry
Author(s) -
Gaskell Simon J.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200150207
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , tandem mass spectrometry , derivatization , chromatography , analytical chemistry (journal) , quadrupole , quadrupole mass analyzer , physics , atomic physics
Dehydroepiandrosterone sulphate (DHAS) has been quantified in human blood serum by fast atom bombardment (FAB)/tandem mass spectrometry of immunoadsorption extracts. FAB of DHAS yielded abundant ions corresponding to the intact steroid sulphate; these were selected by a double‐focusing mass spectrometer prior to collisionally activated decomposition in a quadrupole collision cell and mass analysis by a quadrupole mass filter. [HSO 4 ] − ( m / z 97) was the sole prominent daughter ion. For quantitative analyses the quadrupole mass filter was set to transmit m / z 97 and a narrow‐range magnet scan yielded a spectrum of parents, including m / z 367 and 369, corresponding to DHAS and the ( 2 H 2 )‐analogue (used as internal standard), respectively. Serum concentrations by this procedure were in good agreement with data obtained by gas chromatographic/mass spectrometric analyses of DHA heptafluorobutyrate, formed by direct derivatization of the steroid sulphate.