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Direct analysis of rat bile for acetaminophen and two of its conjugated metabolites via thermospray liquid chromatography/mass spectrometry
Author(s) -
Betowski L. D.,
Korfmacher W. A.,
Lay J. O.,
Potter D. W.,
Hinson J. A.
Publication year - 1987
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200141203
Subject(s) - thermospray , chromatography , acetaminophen , chemistry , mass spectrometry , liquid chromatography–mass spectrometry , conjugated system , tandem mass spectrometry , biochemistry , organic chemistry , selected reaction monitoring , polymer
Bile from rats treated with acetaminophen was analyzed by direct injection onto a thermospray liquid chromatography/mass spectrometry (LC/MS) system. Two conjugated metabolites of acetaminophen were separated by the high‐pressure liquid chromatographic system and analyzed by mass spectrometry. The conjugates were identified as the glutathione–acetaminophen conjugate and the glucuronide–acetaminophen conjugate by comparison of the chromatographic retention times and the mass spectra to that of the synthetic standards. No evidence of acetaminophen metabolites was observed when bile samples were subjected to direct analysis by fast atom bombardment mass spectrometry.