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Quantitative determination of erythromycin 2′‐ethylsuccinate in human plasma by fast atom bombardment mass spectrometry
Author(s) -
Ottoila Pekka,
Taskinen Jyrki
Publication year - 1987
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200141116
Subject(s) - chemistry , detection limit , mass spectrometry , chromatography , calibration curve , analyte , deuterium , diethyl ether , ether , fast atom bombardment , analytical chemistry (journal) , organic chemistry , physics , quantum mechanics
A method for simultaneous quantitative determination of erythromycin 2′‐ethylsuccinate and erythromycin in human plasma is described. After extracting the deuterium‐labelled internal standards and analytes from alkalinized plasma to diethyl ether the extracts were analysed by fast atom bombardment mass spectrometry. The quantification limit for 2′‐ethylsuccinate ester was 50 ng ml −1 and for erythromycin 100 ng ml −1 . The calibration curves were linear up to 5 μg ml −1 for both. The precision of the method at higher concentration was less than 2% and at the quantification limit ∼6% for both analytes.