Premium
Comparison of thermally‐assisted fast atom bombardment (TA‐FAB) with conventional FAB and EI mass spectrometry for the analysis of the Helminthosporium carbonum mycotoxins
Author(s) -
Ackermann B. L.,
Holland J. F.,
Watson J. T.
Publication year - 1987
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200140905
Subject(s) - fast atom bombardment , mass spectrometry , chemistry , atom (system on chip) , analytical chemistry (journal) , chromatography , computer science , operating system
A new technique known as thermally‐assisted fast atom bombardment (TA‐FAB) has been applied to the analysis of a series of cyclic tetrapeptide mycotoxins in order to demonstrate the usefulness of the method for structural elucidation. TA‐FAB uses saturated aqueous solutions of highly hydroxylated compounds, such as fructose, as alternatives to the usual viscous liquid matrices employed in conventional FAB. During the TA‐FAB analysis, the probe tip is resistively heated causing diffeiences to occur in the desorption profiles for the analyte and the matrix ions enabling an optimization for analyte desorption as a function of temperature. In this study, direct comparisons are made between TA‐FAB, conventional FAB, and electron impact ionization for the analysis of the Helminthosporium carbonum mycotoxins at the 1.5 μg level. The results demonstrate the superior capacity of TA‐FAB to provide both molecular weight confirmation and significant fragmentation to aid in the structural elucidation of these important biomolecules.