Premium
Confirmative assay and quantitative determination of Hedera Helix L. saponins (α‐hederin, hederacosides B and C) in raw plant extracts and in cosmetic formulations by EI and CAD MIKE spectrometry
Author(s) -
Facino R. Maffei,
Carini M.,
Traldi P.,
Pelli B.,
Gioia B.,
Arlandini E.
Publication year - 1987
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200140409
Subject(s) - hedera helix , chemistry , mass spectrometry , chromatography , saponin , araliaceae , botany , biology , medicine , alternative medicine , pathology , ginseng
The current methods for the qualitative and quantitative control of cosmetologically active triterpenic saponins in raw plant extracts and in cosmetic formulations are far from satisfactory in terms of specificity and sensitivity. Here we describe a method for the simultaneous detection and quantification of Hedera helix L. saponins directly using crude extracts from plant and cosmetic matrices. The first is achieved by conventional EI‐mass spectral analysis (direct DEI conditions); the second by CAD MIKE spectrometry (collisionally activated decomposition mass‐analysed ion kinetic energy spectrometry) of the EI‐induced fragmentation product at m / z 248, which is common to the three saponins of Hedera helix L. (α‐hederin, hederacosides B and C). The method is sensitive (sub ppb level), specific and since it is rapid it might be conveniently employed for the routine quality control of these active constituents in plant extracts and in cosmetic formulations.