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Acetylation and pentafluorobenzoylation of lidocaine metabolites in aqueous solution and identification of derivatives by combined gas chromatography/mass spectrometry
Author(s) -
Coutts R. T.,
TorokBoth G. A.,
Tam Y. K.,
Chu L. V.,
Pasutto F. M.
Publication year - 1987
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200140407
Subject(s) - chemistry , chromatography , derivatization , mass spectrometry , acetic anhydride , gas chromatography , gas chromatography–mass spectrometry , aqueous solution , amine gas treating , electron ionization , chloride , chemical ionization , organic chemistry , ion , ionization , catalysis
Known and possible phenolic and primary and secondary amine metabolites of lidocaine were added to urine from drug‐naive rats. These metabolites were derivatized in this aqueous medium by acetylation with acetic anhydride or by pentafluorobenzoylation with pentafluorobenzoyl chloride. The derivatives were simultaneously extracted into an organic solvent. The products were separated by gas chromatography (flame ionization detection for acetates and electron‐capture detection for pentafluorobenzoates) and identified by combined gas chromatography/mass spectrometry. Mass spectral fragmentation pathways were readily deduced; diagnostic fragment ions were identified and were valuable for characterization purposes. Structural isomers could be distinguished on the basis of their GC retention times. Extractive derivatization using pentafluorobenzoyl chloride is an attractive analytical procedure for the identification of phenolic and dealkylated metabolites of lidocaine.