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Characterization of an alkylated dinucleotide by desorption chemical ionization and tandem mass spectrometry
Author(s) -
IsernFlecha I.,
Jiang X.Y.,
Cooks R. G.,
Pfleiderer W.,
Chae W.G.,
Chang ChingJer
Publication year - 1987
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200140106
Subject(s) - mass spectrometry , chemistry , tandem mass spectrometry , characterization (materials science) , ionization , chemical ionization , desorption , direct electron ionization liquid chromatography–mass spectrometry interface , analytical chemistry (journal) , alkylation , chromatography , materials science , organic chemistry , nanotechnology , ion , adsorption , catalysis
A highly sensitive (subpicomole level), structure‐specific method of analysis has been developed for characterizing and quantitating the dinucleotide thymidylyl(3′ → 5′)thymidine methyl phosphotriester by desorption chemical ionization tandem mass spectrometry. The methodology can be applied to related compounds such as the parent dinucleotide and employs either positive or negative ionization mass spectra or daughter spectra. The procedure allows specification of the site of methyl attachment.