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Application of plama desorption mass spectrometry to molecular weight determination of structural protein from insect cuticle
Author(s) -
Roepstorff P.,
Højrup P.,
Sundqvist B. U. R.,
Jonsson G.,
Håkansson P.,
Andersen S. O.,
Johansson K. E.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200131209
Subject(s) - locust , mass spectrometry , cuticle (hair) , molecular mass , gel electrophoresis , chemistry , sequence (biology) , desorption , two dimensional gel electrophoresis , polyacrylamide gel electrophoresis , chromatography , biology , biochemistry , proteomics , enzyme , genetics , gene , botany , adsorption , organic chemistry
During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria , a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS‐gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127 I‐plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS‐electrophoresis to be too high.