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Liquid chromatographic‐mass spectrometric analysis of 2‐nitrofluorene and its derivatives
Author(s) -
Möller L.,
Gustafsson JÅ.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200131208
Subject(s) - chemistry , chromatography , high performance liquid chromatography , mass spectrometry , mass spectrum , analytical chemistry (journal)
Liquid chromatography‐mass spectrometry (LC‐MS) with a nebuliser and a moving belt was used to characterise 2‐nitrofluorene, its metabolites and related compounds. In total, 18 different compounds were analysed. The LC‐MS interface did not affect the chromatographic performance achieved by high performance liquid chromatography (HPLC). The method allowed determination of the positions of the hydroxyl groups in different hydroxylated 2‐acetylaminofluorene derivatives. The LC‐MS procedure generated acceptable mass spectra with as small amounts of substance as 65 ng. The dose‐response curves from the HPLC‐detector and the ion‐source in the LC‐MS were parallel and linear.