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High sensitivity assay for the α 2 antagonist 6‐chloro‐3‐methyl‐2,3,4,5‐tetrahydro‐1 H‐3‐benzazepine and its desmethyl metabolite in plasma using gas chromatography mass spectrometry with ammonia/CCl 4 chemical ionization
Author(s) -
Levandoski P.,
Straub K.,
Shah D.,
Demarinis R.,
De Mey C.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200131002
Subject(s) - desmethyl , chemistry , chromatography , metabolite , mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , detection limit , active metabolite , biochemistry
A rapid and highly sensitive assay for quantifying the α 2 ‐adrenoceptor antagonist SK&F 86466 and a desmethyl metabolite has been developed, using capillary column gas chromatography/chemical ionization mass spectrometry. This assay uses deuterium‐labeled analogs of the analytes as internal standards, and has a lower limit of quantification of 100 pg ml −1 . A novel reagent gas, consisting of carbon tetrachloride in anhydrous ammonia, was used to achieve maximal sensitivity.