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Preparation of 18 O‐labelled standards of hydroxy‐eicosatetraenoic acids and thromboxanes for quantitative measurement by gas chromatography/mass spectrometry
Author(s) -
Leis H. J.,
Malle E.,
Moser R.,
Nimpf J.,
Kostner G. M.,
Esterbauer H.,
Gleispach H.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130906
Subject(s) - chemistry , thromboxanes , mass spectrometry , chromatography , gas chromatography , gas chromatography–mass spectrometry , chemical ionization , hydrolysis , derivatization , electron ionization , organic chemistry , ion , ionization , arachidonic acid , enzyme
A study on the extent of 18 O incorporation into various hydroxy‐eicosatetraenoic acids, thromboxane B 2 and 2,3‐dinor‐thromboxane B 2 by base‐catalysed hydrolysis of their methyl ester derivatives is presented. The amount as well as the position of the inbuilt 18 O atoms were determined by gas chromatography/mass spectrometry, utilizing electron impact and positive and negative ion chemical ionization.