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Separation of the components of commercial digitonin using high‐performance liquid chromatography and centrifugal countercurrent chromatography. Identification of the products by californium‐252 plasma desorption mass spectrometry
Author(s) -
Yang Y. M.,
Lloyd H. A.,
Pannell L. K.,
Fales H. M.,
Macfarlane R. D.,
McNeal C. J.,
Ito Y.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130810
Subject(s) - californium , digitonin , chromatography , chemistry , countercurrent chromatography , mass spectrometry , high performance liquid chromatography , biochemistry , membrane , physics , quantum mechanics , neutron
Abstract Commercial digitonin has been separated into its components using high‐performance liquid chromatography and centrifugal countercurrent chromatography. The individual glucosides have been identified by californium‐252 plasma desorption mass spectrometry, occasionally supplemented by hydrolysis and analysis of the liberated sugars and aglycones. Mass spectra of commercial digitonin that have appeared in the literature are discussed in the light of their complex nature. A new glycoside of molecular weight 1035 is described and a structure proposed.