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Analysis of inositol mono‐ and polyphosphates by gas chromatography/mass spectrometry and fast atom bombardment
Author(s) -
Sherman William R.,
Ackermann Karen E.,
Berger Richard A.,
Gish Beverly G.,
Zinbo Mikio
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130704
Subject(s) - inositol , chemistry , fast atom bombardment , mass spectrometry , phosphate , fragmentation (computing) , mass spectrum , inositol phosphate , ion , chromatography , biochemistry , organic chemistry , receptor , computer science , operating system
The electron ionization spectra of all of the positional isomers of myo ‐inositol monophosphate and of myo ‐inositol 1,2‐cyclic phosphate were obtained by gas chromatography/mass spectrometry of the pertrimethylsilyl derivatives. The fragmentation pattern of pertrimethylsilyl myo ‐inositol‐1‐phosphate was studied using deuterium labeling. The phosphate moiety was found to direct fragmentation to produce fragment ions of useful intensity with specific carbon retention. The spectrum of pertrimethylsilyl myo ‐inositol‐1,4‐bisphosphate is also described. An electron impact gas chromatographic/mass spectrometric method for myo ‐inositol‐1‐phosphate has been developed, which has a sensitivity to a level of 0.1 pmol. The positive and negative ion fast atom bombardment spectra of myo ‐inositol hexakis(disodium phosphate) and myo ‐inositol hexakis(dihydrogen phosphate) are described. The lesser‐phosphorylated inositol polyphosphates were also studied, including inositol pentakis and inositol tetrakis(dihydrogen phosphates) as well as D‐ myo ‐inositol‐1,4,5‐trisphosphate and D‐ myo ‐inositol‐1,4‐bisphosphate from human red blood cells. The sensitivity of fast atom bombardment for the measurement of the latter two substances allows their detection to a level of about 10 nmol. The fast atom bombardment spectrum of synthetic myo ‐inositol 1,2‐cyclic phosphate revealed variable amounts of a dimer produced during its preparation.