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Quantitative estimation of quaternary ammonium neuromuscular blocking agents in serum by direct insertion probe chemical ionization mass spectrometry
Author(s) -
Castagnoli K. P.,
Shinohara Y.,
Furuta T.,
Nguyen T. L.,
Gruenke L. D.,
Miller R. D.,
Castagnoli N.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130703
Subject(s) - mass spectrometry , chemistry , ammonium , blocking (statistics) , chromatography , extraction (chemistry) , chemical ionization , neuromuscular blocking agents , deuterium , ion , ionization , anesthesia , organic chemistry , medicine , statistics , mathematics , physics , quantum mechanics
A new method based on selected ion monitoring chemical ionization mass spectrometry was developed to measure the quaternary ammonium neuromuscular blocking agents, pancuronium and vecuronium, in serum. An ion‐pair extraction procedure is utilized to separate the compounds of interest from biological fluids. The intensities of the ion currents produced by the bisamines formed from the drugs and the corresponding deuterated internal standards through thermolytic dequaternization are monitored. The assay shows good linearity over the range of 1–500 ng/ml. This assay has been utilized in a variety of clinical pharmacokinetic studies involving surgical pediatric, geriatric and obstetric patients requiring anesthesia.