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Pyrolysis mass spectrometry of bacteria from infected human urine. I. Influence of culturing and antibiotics
Author(s) -
Huff Stanley M.,
Matsen John M.,
Windig Willem,
Meuzelaar Henk L. C.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130604
Subject(s) - urine , bacteria , chromatography , mass spectrometry , penicillin , chemistry , antibiotics , microbiology and biotechnology , gentamicin , isolation (microbiology) , incubation , biology , biochemistry , genetics
Curie‐point pyrolysis mass spectrometry was performed on three bacterial isolates obtained from infected human urine using a novel, direct isolation method. Bacterial samples were analysed directly after isolation, as well as after free growth in broth and after exposure to solutions containing penicillin and gentamicin. The spectra of bacteria directly from urine showed no detectable contamination from urinary constituents. Discriminant analysis indicated genetic strain differences to be greater than the combined variances due to sample preparation or the growth phase. Characteristic biochemical changes related to growth or non‐growth were detectable after only 2 h of incubation. The potential usefulness of pyrolysis mass spectrometry techniques for rapid susceptibility testing is discussed.