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Quantitative determination of diethylstilboestrol in bovine urine with isotope dilution mass spectrometry
Author(s) -
Jansen E. H. J. M.,
Freudenthal J.,
Van Rossum H. J.,
Litjens J. L. M.,
Stephany R. W.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130507
Subject(s) - isotope dilution , chromatography , chemistry , mass spectrometry , urine , high performance liquid chromatography , dilution , biochemistry , physics , thermodynamics
A method has been described for the quantification of diethylstilboestrol (DES) in bovine urine. The determining step is based on isotope dilution mass spectrometry (IDMS). The chemical work‐up consisted of enzymatic deconjugation, Sep‐Pak® clean‐up and high‐performance liquid chromatography (HPLC). The labelled compound synthesized for IDMS is hexadeutero DES (DES‐ 2 H 6 ). The labelled compound is not only used to quantify DES, but also to check the recovery of the chemical work‐up procedure, in order to avoid false negative results. At the 1–5 μg I −1 level of urinary DES, the average recovery of 2 μg I −1 DES‐ 2 H 6 is 70±20% (s.d.).