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Studies on drug metabolism by use of isotopes XXVIII—biological isotope effect for deuterated ephedrine
Author(s) -
Baba Shigeo,
Kuroda Yumiko,
Horie Masanobu
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130308
Subject(s) - deuterium , isotope , ephedrine , drug , drug metabolism , kinetic isotope effect , chemistry , metabolism , pharmacology , radiochemistry , medicine , biochemistry , physics , nuclear physics
The stability of the deuterium label and the occurrence of an isotope effect for deuterated (—)‐ephedrine during metabolic processes were investigated. Determination of the deuterium content in deuterated (—)‐ephedrine and its metabolites excreted into urine showed that little, if any, deuterium exchange had occurred. In rats, a slight isotope effect was observed for p ‐hydroxylation (about 15%) and glucuronide formation of p ‐hydroxyephedrine and/or its enzymatic hydrolysis (about 20%). In human subjects, there was about 15% difference in the excretion of norephedrine between deuterated and non‐deuterated species.