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An assay for paracetamol, produced by the O‐deethylation of phenacetin in vitro , using gas chromatography/electron capture negative ion chemical ionization mass spectrometry
Author(s) -
Murray S.,
Boobis A. R.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130208
Subject(s) - chemistry , chromatography , mass spectrometry , chemical ionization , ion , microsome , detection limit , phenacetin , electron ionization , gas chromatography , ionization , analytical chemistry (journal) , in vitro , organic chemistry , biochemistry
Abstract A sensitive gas chromatographic/mass spectrometric assay for paracetamol, produced by the O ‐deethylation of phenacetin by microsomal fractions of human liver, has been developed. The method uses the 3,5‐bistrifluoromethyl‐benzoyl derivative of paracetamol and electron capture negative ion chemical ionization. This derivative has a negative ion mass spectrum which contains only a molecular ion at m/z 391 and, when this ion is specifically monitored, an amount of derivative equivalent to 1 pg of paracetamol can be detected. ( 2 H 3 )Paracetamol was available for use as an internal standard and this allowed the development of an assay with a precision of 8% (SD) at 0.5 ng paracetamol per microsomal incubation and a lower limit of quantitative determination of 0.2 ng paracetamol per microsomal incubation.