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Electron capture negative chemical ionization mass spectrometry of chemically oxidized corticosteroids
Author(s) -
Her G. R.,
Watson J. T.
Publication year - 1986
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200130203
Subject(s) - mass spectrometry , chemical ionization , chemistry , electron ionization , ionization , electron capture , ion , chromatography , organic chemistry
A procedure which can be used to increase the electron affinity of corticosteroids for purposes of quantitative analysis is porposed. Ten important corticosteroids are chemically oxidized to 1,4‐androstadien‐3,11,17‐trione or 4‐androsten‐3,6,17‐trione structural steroid analogs to enhance their electrophilicity prior to detection by electron capture negative chemical ionization mass spectrometry. The choice of oxidation reagent, a preliminary assessment of the structure‐response relationship of the oxidation products, the optimization of negative ion formation as a function of source temperature and the basis for distinguishing the isomers dexamethasone and betamethasone are addressed.