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Direct mass spectrometric determination of 13 C enrichment of organic compounds
Author(s) -
Liu R. H.,
Smith F. P.,
Low I. A.,
Piotrowski E. G.,
Damert W. C.,
Phillips J. G.,
Liu J. Y.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200121103
Subject(s) - chemistry , natural abundance , chemical composition , isotope , analytical chemistry (journal) , ion , mass spectrometry , chromatography , abundance (ecology) , isotopic ratio , organic chemistry , physics , quantum mechanics , fishery , biology
Abstract A direct mass spectrometric measurement method is developed for the determination of 13 C enrichment and chemical purity of a labelled compound. The labelled compound is mixed with various amounts of unlabelled analogue to provide a series of mixtures containing different but comparable quantities of labelled and unlabelled species. Intensity ratios of selected isotopically anologous ions from these two species are determined using selected ion monitoring. Equations are established to relate measured intensity ratios to sample mixture weight ratios, chemical purities of the labelled and unlabelled compounds, ion composition and isotopic abundance of the enriched and unenriched carbons, A non‐linear regression procedure, based on the measured intensity ratio, the mixture weight ratio and the known chemical purity of the unlabelled compound, is used to resolve the chemical purity and the isotopic abundance yielding results with standard errors of 1.0 and 0.4%, respectively. This procedure is suitable for compounds labelled with more than 60% of an isotope.