Premium
Selected ion monitoring mass spectrometry: Parameters affecting quantitative determination
Author(s) -
Low I. A.,
Liu R. H.,
Barker S. A.,
Fish F.,
Settine R. L.,
Piotrowski E. G.,
Damert W. C.,
Liu J. Y.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200121102
Subject(s) - mass spectrometry , ion , calibration , selected ion monitoring , analytical chemistry (journal) , dwell time , chemistry , hydrogen , chromatography , physics , gas chromatography–mass spectrometry , medicine , clinical psychology , organic chemistry , quantum mechanics
Instrumental parameters and possible correction procedures for hydrogen‐transfer phenomena are studied, with emphasis on the improvement of quantitative measurements in selected ion monitoring mass spectrometry. The intensity ratio of the m / z 387 and 386 ions, derived from cholesterol, are measured by an HP‐5995 mass spectrometer using different parameters, including calibration peak width, mass filter window size and ion monitoring dwell time. The intrinsic ratio of these two ions is calculated with a correction procedure accounting for the [MH] + and [M2H] + hydrogen‐transfer phenomena.