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Rapid and efficient sequence determination of a 4 nmol sample of adipokinetic hormone I (AKH I) using FAB tandem mass spectrometry
Author(s) -
Eckart Klaus,
Schwarz Helmut,
Ziegler Rolf
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200121010
Subject(s) - adipokinetic hormone , tandem mass spectrometry , mass spectrometry , chromatography , chemistry , sequence (biology) , biochemistry , fat body , gene
Fast atom bombardment mass spectrometery in conjunction with tandem mass spectrometry provided the unambiguous sequence determination of the adipokinetic hormone I. The amount of sample needed is of the order ⩽4 nmol, and the complete analysis is performed in less than 4 h.