Structural investigations on the inner core region of lipopolysaccharides from  Salmonella minnesota  rough mutants | Zendy

Brade Helmut | Zendy; Moll Hermann | Zendy; Rietschel E. Th. | Zendy

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Structural investigations on the inner core region of lipopolysaccharides from Salmonella minnesota rough mutants

Author(s) -

Brade Helmut,

Moll Hermann,

Rietschel E. Th.

Publication year - 1985

Publication title -

biomedical mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0306-042X

DOI - 10.1002/bms.1200121007

Subject(s) - mutant , chemistry , tetrasaccharide , salmonella , sugar , mass spectrometry , core (optical fiber) , inner core , strain (injury) , stereochemistry , biochemistry , chromatography , biology , bacteria , genetics , materials science , polysaccharide , gene , anatomy , composite material

The structure of the inner core region ( L ‐glycero‐ D ‐mannoheptose/2‐keto‐3‐deoxy‐ D ‐mannooctulosonic acid region) of lipopolysaccharides from Salmonella minnesota rough mutants was investigated. Using conventional methods (neutral sugar analysis, Smith degradation and methylation analysis) combined with gas chromatography/mass spectrometry (GC/MS) of higher oligosaccharides (up to tetrasaccharide), the linkages of the core sugars of lipopolysaccharides from S. minnesota rough mutants, strains R4 (Rd 2 P − ), R7 (Rd 1 P − ) and R5 (RcP − ) were determined as:with R representing H in R4, L‐glycero‐D‐mannoheptopyranosyl in R7, and D‐glucopyranosyl‐(1→3)‐ L ‐glycero‐ D ‐mannoheptopyranosyl in R5, respectively. In addition, it is shown that heterogeneity within the neutral sugar part of these lipopolysaccharides is low.

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