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Gas chromatographic negative ion mass spectrometric assay of 2‐dicyclopropylmethylamino‐2‐oxazoline (S‐3341), a new antihypertensive drug
Author(s) -
Ehrhardt J. D.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200121005
Subject(s) - chromatography , oxazoline , chemistry , mass spectrometry , organic chemistry , catalysis
Abstract The quantification in plasma and urine of 2‐dicyclopropylmethylamino‐2‐oxazoline (S‐3341), a new antihypertensive drug is described using a sensitive gas chromatographic negative ion mass spectrometric method with ammonia as moderating gas. After a two‐step extraction, derivatization is carried out with 3,5‐bis(trifluoromethyl)benzoyl chloride and the abundance of the molecular ion ( m / z 420) obtained is compared with that of the tetradeuterated standard ( m / z 424). The low background due to the high mass and negative ion detection provides a detection limit of about 1 pg per injection. Oral administration of 1 or 2 mg S‐3341 to patients gives a maximum concentration of 3.3±0.7 ng ml −1 and 7.6±2.0 ng ml −1 at 1.8±0.6 h and 1.4±0.7 h and an average elimination half‐life of 6.7 h.