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Evaluation of fast atom bombardment mass spectrometry for assessing the oxidation states of disulfide‐containing peptides
Author(s) -
Larsen B. S.,
Yergey J. A.,
Cotter R. J.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200121003
Subject(s) - fast atom bombardment , chemistry , ion , atom (system on chip) , mass spectrometry , mass spectrum , analytical chemistry (journal) , disulfide bond , natural abundance , chromatography , organic chemistry , computer science , embedded system , biochemistry
Positive and negative ion fast atom bombardment (FAB) mass spectra of the molecular ion region of calcitonin using several solution matrices produce an isotopic distribution which is indicative of a mixture of reduced and oxidized disulfide bonds. The insensitivity of the distribution to the choice of matrix and positive/negative ion mode suggest that the spectra reflect the natural abundance of reduced calcitonin in the sample, rather than an artifact of the fast atom bombardment technique.