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Hydroxyl negative chemical ionization mass spectrometry linked with collisionally activated decomposition. A modern analytical tool in inborn errors of metabolism
Author(s) -
Rinaldo P.,
Miolo G.,
Chiandetti L.,
Zacchello F.,
Daolio S.,
Traldi P.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120922
Subject(s) - chemistry , mass spectrometry , chemical ionization , decomposition , chromatography , ion , ionization , gas chromatography–mass spectrometry , analytical chemistry (journal) , organic chemistry
Two kinds of inborn errors of metabolism, dicarboxylic aciduria and hyperoxaluria, have been studied by means of hydroxyl negative ion chemical ionization [NICI(OH − )], linked with collisionally activated decomposition experiments on the [MH] − species of the pathognomonic organic acids. This method has led to non‐controversial qualitative determinations of C4–C10 dicarboxylic acids and oxalic, glyceric and glyoxylic acids. NICI(OH − ) linked with collisionally activated decomposition mass analysed ion kinetic energy spectrometry (CAD MIKES) is proposed herein for diagnostic purposes, as a valid mass spectrometric alternative to standard gas chromatographic/mass spectrometric analysis. The procedure is characterized by simplified sample treatment and by fast execution.