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Keto acids in tissues and biological fluids: O‐t‐ butyldimethylsilyl quinoxalinols as derivatives for sensitive gas chromatographic/mass spectrometric determination
Author(s) -
Langenbeck U.,
Luthe H.,
Schaper Gritta
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120912
Subject(s) - chemistry , trimethylsilyl , mass spectrum , electron ionization , mass spectrometry , chromatography , gas chromatography , chemical ionization , selected ion monitoring , ion , ionization , biological fluids , polyatomic ion , gas chromatography–mass spectrometry , analytical chemistry (journal) , organic chemistry
Quinoxalinol t ‐butyldimethylsilyl ethers were prepared from three branched‐chain and from two aliphatic unbranched 2‐keto acids. The electron impact (EI) mass spectra display pronounced [M −57] + ions. With 39–51% of total ion current contained within them, sensitivity is greater than on chemical ionization (CI) mass spectrometry of O ‐trimethylsilyl derivatives. Mass spectra and chromatographic behaviour of these novel keto acid derivatives are discussed and preliminary quantitative data from rat muscle are given.