Analysis of (1‐ 13 C)leucine and ( 13 C)KIC in plasma by capillary gas chromatography/mass spectrometry in protein turnover studies | Zendy

Ford G. C. | Zendy; Cheng K. N. | Zendy; Halliday D. | Zendy

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Analysis of (1‐ 13 C)leucine and ( 13 C)KIC in plasma by capillary gas chromatography/mass spectrometry in protein turnover studies

Author(s) -

Ford G. C.,

Cheng K. N.,

Halliday D.

Publication year - 1985

Publication title -

biomedical mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0306-042X

DOI - 10.1002/bms.1200120814

Subject(s) - chemistry , leucine , chromatography , metabolite , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , selected ion monitoring , chemical ionization , amino acid , ion , ionization , biochemistry , organic chemistry

The methods used for the determination of the concentration and isotope enrichment of (1‐ 13 C)leucine and its metabolite ( 13 C)α‐ketoisocaproic acid (KIC) in plasma for the study of whole‐body protein turnover are described. Leucine was analysed as its N ‐heptafluorobutyryl isobutyl ester and KIC as its quinoxalinol‐TMS derivative, both by chemical ionization selected ion monitoring gas chromatography/mass spectrometry (GC/MS). The sensitivity of the leucine assay was improved 30 times by monitoring the negative ions under the conditions described. The coefficient of variation for enrichment and concentration measurements were 0.5% and 2%, respectively, with a minimum detectable enrichment of 0.1at% excess for both assays.

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