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Determination of infused ( 13 C)progesterone in ovarian arterial blood by selected ion monitoring
Author(s) -
Halket John M.,
Leidenberger Freimut,
EinerJensen Niels,
Bendz Arne
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120813
Subject(s) - ovarian vein , ovary , in utero , hormone , ovarian artery , vein , arterial blood , endocrinology , steroid hormone , endogeny , hysterectomy , medicine , venous blood , selected ion monitoring , in vivo , steroid , chemistry , biology , gas chromatography–mass spectrometry , mass spectrometry , uterine artery , chromatography , fetus , pregnancy , pathology , gestation , microbiology and biotechnology , genetics
Gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM) is employed to detect and approximate the levels of ( 13 C)progesterone in small blood samples obtained at intervals from ipsilateral and contralateral ovarian arteries and a peripheral vein before, during and after infusion into the utero‐ovarian vein (five women during hysterectomy). The internal standard was 16α‐methylprogesterone. The method is rapid and allows the time course of the infused hormone to be plotted in spite of large and rapidly fluctuating concentrations of endogenous progesterone. The results show for the first time the local in vivo transfer of a steroid hormone from the uterine vein to the adjacent ovary in humans.