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Mass‐Analysed ion kinetic energy spectra and B 1 E–B 2 triple sector mass spectrometric analysis of phosphoinositides by fast atom bombardment
Author(s) -
Sherman William R.,
Ackermann Karen E.,
Bateman Robert H.,
Green Brian N.,
Lewis Ivor
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120810
Subject(s) - fast atom bombardment , ion , chemistry , kinetic energy , mass spectrum , phosphatidic acid , isobaric process , analytical chemistry (journal) , mass spectrometry , fatty acid , spectral line , phospholipid , chromatography , organic chemistry , biochemistry , thermodynamics , physics , quantum mechanics , astronomy , membrane
Fast atom bombardment is shown to produce useful spectra of the three phosphoinositides and the metabolically related phospholipids, lysophosphatidylinositol and phosphatidic acid. Analysis of the [MH] − ions for fatty ester composition by mass‐analysed ion kinetic energy spectra (MIKES) is shown to be inadequate to resolve fatty acyl daughter ions when the parent ion contains isobaric species. However, analysis on a triple sector instrument with and without collisional activation does provide complete compositional information. Quantitative analysis of the fatty ester content of each lipid molecular species is complicated by dissimilar ion yields from fatty acyl‐bearing fragments from compositionally different parent ions.