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Identification of 17α, 20α‐dihydroxyprogesterone in testicular extracts after incubation with ketoconazole
Author(s) -
Lauwers W. F. J.,
Jeune L. Le,
Bossche H. Vanden,
Willemsens G.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120608
Subject(s) - ketoconazole , incubation , derivatization , testosterone (patch) , chromatography , endocrinology , medicine , chemistry , biology , antifungal , biochemistry , high performance liquid chromatography , microbiology and biotechnology
The antifungal ketoconazole affects testosterone synthesis in dispersed rat testicular cells. In the presence of ketoconazole an accumulation of 17α,20α‐dihydroxyprogesterone has been observed. This steroid was isolated from the testis of Wistar rats after a [4‐ 14 C]progesterone incorporation in the presence of ketoconazole. Its identification was achieved from the gas chromatographic/mass spectrometric analysis of the isolated radioactive fraction. A chemical derivization of the fraction with butylboronic acid followed by mass spectrometric analysis confirmed the presence of 17α,20α‐dihydroxyprogesterone.