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Quantitative secondary ion monitoring gas chromatography/mass spectrometry of diethylstilbestrol in bovine liver
Author(s) -
Covey Thomas,
Maylin George,
Henion Jack
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120606
Subject(s) - chromatography , mass spectrometry , chemistry , selected ion monitoring , gas chromatography–mass spectrometry , diethylstilbestrol , gas chromatography , extraction (chemistry) , divinylbenzene , organic chemistry , hormone , styrene , biochemistry , copolymer , polymer
A procedure is described for the extraction of diethylstilbestrol (DES) from animal tissue for quantitative capillary gas chromatography/mass spectrometry (GC/MS). The procedure is based upon use of a strong anion exchange polystyrene divinylbenzene resin for sample purification. The recovery of DES from the resin clean up was 88% in the high parts per trillion (ppt) range. Criteria for identification of DES using selected ion monitoring (SIM) GC/MS are presented. Liquid chromatography/mass spectrometry (LC/MS) was used to investigate altered DES cis/trans ratios observed in biological extracts.