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Comparison of gas chromatographic/mass spectrometric and microbiological assays for 5‐fluorouracil in plasma
Author(s) -
Min Bo H.,
Garland William A.,
Lewinson T. M.,
Mehta Bipin M.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120510
Subject(s) - chromatography , chemistry , correlation coefficient , gas chromatography , mass spectrometry , fluorouracil , analytical chemistry (journal) , biology , mathematics , cancer , genetics , statistics
The concentrations of 5‐fluorouracil in 99 plasma samples were determined by both microbiological and gas chromatographic/mass spectrometric assays. The values determined by the two methods were similar (correlation coefficient = 0.90). A regression of the natural logarithms of the concentrations (0.01–94 μg ml −1 ) determined by the two methods gave a line whose slope and intercept were not statistically different (p>0.05) from 1 and 0 respectively. Thus, the microbiological assay has specificity over a sufficient concentration range to serve as a practical routine laboratory method for the determination of 5‐fluorouracil.