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Gas chromatographic/mass spectrometric analysis of 19‐nortestosterone urinary metabolites in man
Author(s) -
Massé Robert,
Laliberté Claude,
Tremblay Lyne,
Dugal Robert
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120305
Subject(s) - chromatography , chemistry , selected ion monitoring , metabolite , urine , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , trimethylsilyl , nandrolone , organic chemistry , biochemistry , anabolism
A sensitive and highly specific method based on capillary column gas chromatography/mass spectrometry has been developed for the detection of 19‐nortestosterone (17β‐hydroxy‐4‐estren‐3‐one) metabolites in urine. After intramuscular administration of 19‐nortestosterone decanoate to man, urine samples were collected during several days and treated with Helix pomatia digestive juice. The free steroids were extracted and converted into O ‐methyl‐oxime‐trimethylsilyl or the trimethylsilyl ether derivatives and analysed by capillary column gas chromatography/mass spectrometry (GC/MS). Three isomeric metabolites were detected and identified as 3α‐hydroxy‐5α‐extran‐17‐one (19‐norandrosterone), 3α‐hydroxy‐5β‐estran‐17‐one (19‐noretiocholanolone) and 3β‐hydroxy‐5α‐estran‐17‐one (19‐norepiandrosterone). Packed column GC/MS was also employed in the selected ion monitoring mode for the specific detection of 19‐norandrosterone, the most abundant urinary metabolite of 19‐nortestosterone. These gas chromatographic/mass spectrometric methods are highly specific tests which can be used on a routine basis for the confirmation of 19‐nortestosterone administration to athletes as well as for therapeutic monitoring following administration of the drug.