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A stable isotope assay for phenprocoumon and its metabolites
Author(s) -
Heimark L. D.,
Trager W. F.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120204
Subject(s) - phenprocoumon , chemistry , diazomethane , chromatography , detection limit , resolution (logic) , gas chromatography , mass spectrometry , microsome , organic chemistry , enzyme , medicine , artificial intelligence , computer science , warfarin , cardiology , atrial fibrillation
A gas chromatographic/mass spectrometric assay for quantifying phenprocoumon and its 4′‐, 6‐, 7‐ and 8‐hydroxy metabolites in microsomal preparations is described. This assay which uses deuterium‐labeled analogs of the phenprocoumon metabolites as internal standards has a lower limit of quantitation of 20 ng ml −1 . Diazomethane is used to derivatize both metabolites and parent compound yielding along with the expected 4‐methoxy derivative a minor amount of the 2‐methoxychromone. Resolution of the methylated metabolites is accomplished by capillary gas chromatography.