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Measurement of propericiazine in plasma by capillary gas chromatography and selected ion monitoring detection
Author(s) -
De Leenheer André P.,
Jonckheere Jozef A.,
Lefevere Marc F.,
De Broe Marc E.,
Verpooten Gert A.
Publication year - 1985
Publication title -
biomedical mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0306-042X
DOI - 10.1002/bms.1200120106
Subject(s) - chromatography , chemistry , acetic anhydride , derivatization , extraction (chemistry) , gas chromatography , selected ion monitoring , hexane , capillary action , analyte , gas chromatography–mass spectrometry , mass spectrometry , materials science , organic chemistry , composite material , catalysis
A new method, based upon a selective extraction and gas chromatographic/mass spectrometric analysis, was developed to monitor the effect of combined haemoperfusion‐haemodialysis treatment in a case of propericiazine poisoning. The method relies on the selected ion monitoring of the acetate derivatives of propericiazine and its internal standard fluphenazine, after their extraction from 1 ml of alkalinized plasma with n ‐hexane: isopropanol (8:2, v/v), back‐extraction into an acidified water phase, realkalinization and extraction with n ‐hexane:isopropanol, derivatization with acetic anhydride and gas chromatography on a short (12 m) OV‐101 fused silica capillary column. The described procedure is specific and provides between‐assay variability of 4.8% CV at 5 μg I −1 plasma concentration. The method enables quantification down to 1 μg I −1 and hence demonstrates sufficient sensitivity to permit pharmacokinetic or drug monitoring studies.